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ball milll koto drgee angle gora

May 01, 2016· Seedlings were flash frozen in liquid nitrogen and lyophilized. Dried material was beaten with three steel balls in an MM 400ball mill(Retsch) twice for 2 min at 25 Hz. Balls were removed, and the powdered material was then washed twice with 70% (v/v) ethanol, three times with 1:1 (v/v) chloroform:methanol, and once with acetone.

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